[Anubis]

Taken early this morning in the bush. Not as happy with the sharpness as I'd hoped but I've only had my camera 2 days

[NT73]

Take a second shot stopped down and amalgamate them.

[Rabid Penguin]

looks like you got the edges in focus instead of the middle.

[Keith Alan]

Using a much smaller aperture would help--when you get this close to the lens the depth of focus becomes very shallow and you need all the help you can get.
(This is why I always use off-camera flash for macros now).

With ambient light, as long as the shutter speed doesn't get to be too long you'll be fine.
But if it gets into the danger-zone for hand-held, or subject-blur if the wind is blowing, you'll need to add light.
Even a reflector of some kind can make a big difference.
And going to iso400 may have helped give you some added exposure value.

When it comes to focus and macros, moving your focus-point brackets around really helps.
Try to select a focus point that you think will be the most beneficial to the subject.
In this case, that would probably be 1/3rd of the way up from the center since acceptable depth of focus is usually twice as deep behind the focus point compared to in front of it.

You may need to employ the "focus, recompose" strategy to get good results.

Hope this helps.

[Anubis]

Thank you for that advice - very helpful